ZytoLight ® HER2 FISH / SPEC ERBB2/TOP2A/CEN 17 Triple Color Probe

 The ZytoLight ® SPEC ERBB2/TOP2A/ CEN 17 Triple Color Probe is designed for the simultaneous detection of ERBB2 and TOP2A gene status. The ERBB2 gene (a.k.a. HER2 and NEU) is located in the chromosomal region 17q12 and encodes a 185 kDa transmembrane glycoprotein. The TOP2A (topoisomerase II alpha) gene is located in the chromosomal region 17q21.2 and encodes a 170 kDa DNA topoisomerase. The TOP2A gene is frequently either co-amplified or deleted in ERBB2 positive breast cancer cases. TOP2A functions as the target for several anticancer agents, e.g. anthracyclines. Recent data suggests that amplification and deletion of the TOP2A gene locus may account for relative chemosensitivity or resistance to TOP2A inhibitor therapy in ERBB2 positive breast cancer. Thus, determination of the ERBB2 and TOP2A status may help to predict benefit from adjuvant anthracyclines in breast cancer treatment.