5x HOT FIREPol Probe Universal qPCR Mix is optimized for real-time quantitative PCR assays and contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol DNA Polymerase supplied in a proprietary reaction buffer that enables efficient amplification of AT-rich, regular and GC-rich (up to 75%) targets. HOT FIREPol Probe Universal qPCR Mix is optimized for DNA/LNA hydrolysis probes based on the 5´–3´ exonuclease activity.
HOT FIREPol DNA Polymerase is activated by a 10 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
8 ml
